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Volume 12
Journal of Molecular and Genetic Medicine
ISSN: 1747-0862
May 21-23, 2018 Barcelona, Spain
&
Integrative Biology
6
th
International Conference on
Genomics and Molecular Biology
10
th
International Conference on
Genomics 2018 and Integrative Biology 2018
May 21-23, 2018
JOINT EVENT
SPARC enhance acute corneal repair after chemical injury in a rat dry eye model
Yun-Ching Cheng
1
, Wen-Yang Lai
1
, Wan-Yu Hsieh
2
and
Shu-Ching Hsu
3
1
Chang Bing Show Chwan Memorial Hospital, Taiwan
2
National Cheng Kung University, Taiwan
3
National Institute of Infectious Diseases and Vaccinology, Taiwan
Objective:
The objective of this study was to report the evaluation of efficacy of mesenchymal stem cells (MSC) conditional
medium for the treatment of severe keratitis in dry eye disease in early-access program. Unique abilities of MSC could be used
to develop new treatment approaches for dry eye disease.
Materials &Methodology:
An SD rat dry eye model was used in which chemical damage corneal break decreased the Schirmer
test score by at least 40%. The eye symptom score, breakup time of tear film (BUT) and Schirmer test score were compared
before and after treatment in the two groups (MSC conditional medium in normaxia and hypoxia). The repair ability of
conditional medium of MSC by wound weal assay using HUVEC and cancer cell line. We identified the specific component
of hypoxia conditional medium of WJ-MSC (Wharton’s jelly) by mass spectrometry (MS-MS). Classification of specific high
expression protein was determined by western blot analysis.
Results & Discussions:
Hypoxia conditional medium of WJ-MSC showed significant high repair ability than normaxia
conditional medium of WJ-MSC in SD rat dry eye model. SPARC was identified as major protein in hypoxia conditional
medium. According to the different days of conditional medium by western blot analysis, SPACR (secreted protein, acidic and
rich in cysteine) was increased as time-dependent manner. Cell mobile ability was being increase by SPACR in serum-free
culture condition of HUVEC. In SD rat dry eye model, rat continuing treatment with PBS, Artificial tears and SPACR for 2
weeks and determine the recovery by BUT and Schirmer test score. SPACR show the best recovery ability than others.
Conclusions:
In a rat dry eye model, SPARC, the special expression in hypoxia conditional medium, show great recovery
ability. Cell based assay, SPARC enhance the cell wound weal. According these results, we consider that SPARC is a potential
therapeutic agent for use in the treatment of dry eye syndrome.
Biography
Yun-Ching Cheng has completed her PhD from National Sun Yat-sen University and Post-doctoral studies from Cancer Research of NHRI in Taiwan. Her studies
focus on the role of hypoxia condition-expression of proteins/genes in cancer metastasis and the repair ability of hypoxia-induced proteins in MSC. She has
published more than five papers in reputed journals.
greentree67123@gmail.comYun-Ching Cheng et al., J Mol Genet Med 2018, Volume 12
DOI: 10.4172/1747-0862-C2-028