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Volume 12
Journal of Molecular and Genetic Medicine
ISSN: 1747-0862
May 21-23, 2018 Barcelona, Spain
&
Integrative Biology
6
th
International Conference on
Genomics and Molecular Biology
10
th
International Conference on
Genomics 2018 and Integrative Biology 2018
May 21-23, 2018
JOINT EVENT
Bridging pheno-plasticity with genetic profile of the hydrophyte
Ludwigia
stolonifera (Guill. & Perr.)
P.H. Raven: With reference to its expansion to new habitats
Azza Badr Hamed
and
Wafaa M Amer
Cairo University, Egypt
L
udwigia
L is a pantropic genus of aquatic and subaquatic herbs. Recently, L. stolonifera (Guill. & Perr.) P.H. Raven became a
dominant aquatic macrophyte in Egypt and it expanded from fresh water into salty habitats. Interestingly, the plant showed
morphological plasticity in several characters such as leaf shapes, vesicles, flower sex, fertilization efficiency, fruit parameters
and number of produced seeds/fruit. Accordingly, in Egypt this species morphologically grouped into seven morphotypes
(1–7). The lack of information about the plant genome further complicates the identification of these morphotypes. Thus, it
was crucial to investigate the morphotypes in terms of karyotyping and genetic profile to understand if this morphological
plasticity is genetically based or it is an impression of habitat diversity. In this study, seven morphotypes located in different
Egyptian habitats were compared using karyotyping and random amplified polymorphic DNA (RAPD-PCR) technique.
Karyotyping indicated that some morphotypes were tetraploid (2n=32), while others morphotypes were triploid (2n=24).
The measured similarity percentage based on RAPD data revealed a highest value (98.6%) between the triploid M4 and M5
morphotypes; similarly between the tetraploid M1 and M2 morphotypes showed 95.4%. Meanwhile, the lowest similarity
(73.4%) was between the aquatic morphotype (M2) and the salt affected wetland morphotype (M3). The possible link between
the genetic composition and ecological variation of this phenoplastic species will be discussed further.
azza.hamed56@yahoo.comJ Mol Genet Med 2018, Volume 12
DOI: 10.4172/1747-0862-C2-028