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.com
Volume 12
Journal of Molecular and Genetic Medicine
ISSN: 1747-0862
May 21-23, 2018 Barcelona, Spain
&
Integrative Biology
6
th
International Conference on
Genomics and Molecular Biology
10
th
International Conference on
Genomics 2018 and Integrative Biology 2018
May 21-23, 2018
JOINT EVENT
Danshensu rescues ischemia/reperfusion caused hepatocyte damage
Chun Ya Liang
1
, Maw Sheng Sun
1
and
Chan Yen Kuo
2, 3
1
Show Chwan Memorial Hospital, Taiwan
2
National Central University, Taiwan
3
Hsin Sheng Junior College of Medical Care and Management,Taiwann
Introduction:
Apoptosis of hepatocyte, under ischemia/reperfusion (IR) conditions, has been identified as an essential process
in the progression of liver transplantation. Under these conditions, mitochondria can become a threat to the cell because of
their capacity to generate reactive oxygen species (ROS). Additionally, ROS overproduction may induce inflammation. As ROS
accumulation appear to cause hepatocyte damage or death, there has been considerable interest in identifying the candidate
natural products involved and in developing strategies to reduce oxidative stress.
Materiasl & Methods:
In this study, we use Danshensu as an candidate product to speculate whether has the protective effect
on apoptotic hepatocyte upon IR. To speculate the apoptotic phenomena was reversed by Danshensu, we detected the p53,
cleaved-caspase 3 expression by western blotting, as well as caspase-3 activity. Additionally, we analyzed the ROS levels by 2'
,7'-dichlorofluorescin diacetate (DCF-DA) staininng. We also detected the cell viability by WST-1.
Results & Discussion:
Results showed that Danshensu alleviated hypoxia-caused cell apoptosis via ROS overproduction.
However, the precise roles of ROS in liver as a regulatory, protective, or deleterious mediator are still unresolved questions and
need to be further investigated.
Conclusion:
We suggested that Danshensu is a good strategy for treating hepatocyte damage upon IR.
Biography
Yi-Ru Ho completed her Graduation from Department of Molecular Biology and Human Genetics, Tzu Chi Universit. Currently, she is a Research Assistant in
Department of Medical Research Chang Bing Show Chwan Memorial Hospital. She is interested in “Primary cell culture of ADMSC, cell culture of neuron cell, MTT
assay, DNA extraction, qPCR, Western blot, flow cytometry of cell cycle and cell marker, Luciferase assay, siRNA transfection, Enzyme-linked immunosorbent
assay, intraperitoneal injection of mice”.
cynthia1006@gmail.comChun Ya Liang et al., J Mol Genet Med 2018, Volume 12
DOI: 10.4172/1747-0862-C2-028