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Volume 7
Molecular Biology: Open Access
Molecular Biology Meet 2018
August 27-28, 2018
August 27-28, 2018 Dubai, UAE
Molecular Biology and Medicine
International Conference on
Investigating of antibacterial effect of Savory under NaCl and SA treatments
Zahra Aryan
1
, Hassan Ebrahimzadeh
1
, Javad Hadian
2
, Masoud Mirmasoumi
1
and Mahdi Rahaie
1
1
University of Tehran, Iran
2
Shahid Beheshti University, Iran
S
avory
(Satureja khuzistanica)
is a medicinal plant from the Labiatae family. It has antifungal and antibacterial properties due to
phenolic compounds in its essential oils and tannins in the leaves. The purpose of this study is to investigate the antibacterial
properties of leaf and stem extracts of
Satureja
using conventional microbiological methods (measuring diameter of inhibition zone).
Also, the effect of salinity and salicylic acid on phenolic components (Rosmarinic acid and Cafeic acid) and relative
RAS
gene
expression were investigated. The experiment was carried out with two replications and treatment levels of NaCl (100 and 200 mM)
with SA concentration (0.05 Mm). Apical part was tissue cultured on Murashige and Skoog medium and then different salinity levels
and SA treatment were treated for 14 days. The methanolic extract impregnated discs was affected on Muller-Hinton agar medium
containing the bacteria. The applied bacteria in this study includes
Pseudomonas aeruginosa
(ATCC, 27583),
Escherichia coli
(ATCC,
25922),
Enterococcus faecalis
(ATCC, 25923) and
Staphylococcus aureus
(ATCC, 25923). In conclusion, the medicinal components
increased under NaCl and SA treatments. Moreover, relative
RAS
gene expression showed the same results as well. The experiment
showed that methanolic extract has antimicrobial effects on
Escherichia coli, Enterococcus faecalis
and
Staphylococcus aureus
that
would increase under NaCl and SA treatments.
Staphylococcus aureus
and Escherichia coli was formed in inhibition zone with
further diameter. The results of experiments showed that Savory can be used as a medicine plant in the treatment of infections due to
Staphylococcus aureus
and
Escherichia coli.
zahra.aryan@alumni.ut.ac.irMol Biol 2018, Volume 7
DOI: 10.4172/2168-9547-C1-003