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Volume 7

Molecular Biology: Open Access

Molecular Biology Meet 2018

August 27-28, 2018

August 27-28, 2018 Dubai, UAE

Molecular Biology and Medicine

International Conference on

Characterization and pathological variability of

Exserohilum turcicum

responsible for causing

Northern Corn Leaf Blight (NCLB) disease in Malaysia

Abdulaziz Bashir Kutawa

1,2

, Kamaruzaman Sijam

and Khairulmazmi Ahmad

Federal University Dutsin-Ma, Nigeria

University Putra Malaysia, Malaysia

orn is grown primarily for human consumption. It is considered as the second most important cereal crop after rice in Asia.

Many diseases affect this crop due to planting of susceptible hybrids. This research is aimed to characterize the causative agent

of northern corn leaf blight disease in Malaysia, caused by

Exserohilum turcicum

. Leaf samples were collected from infected farms

of two corn growing areas of Peninsular Malaysia in 2015. A total of 5 fungal isolates were examined for cultural, morphological

and molecular properties, the five isolates were identified as

E. turcicum

. The conidial shapes were observed to be elongated and

spindle. Cultural characteristics showed that variation existed among the isolates in colony growth and color. Mycelia growth

rates of the isolates were significantly different on Potato Dextrose Agar (PDA), Corn Meal Agar (CMA) and Potato Sucrose Agar

(PSA) media; growth on CMA was faster than on PSA and PDA. The isolates were grouped into three groups based on colony

color i.e. light gray, gray and dark gray. The isolates were categorized into two groups based on growth namely, moderate growth

and profuse growth. The number of septa ranged from 5-7 to 7-10 representing isolates ET002 and ET003, respectively. Similarly,

conidial length varied from 56.7 μm to 89.44 μm for isolates ET002 and ET003, respectively. The pathogenic variability tested on

Thai Super Sweet (TSS) corn variety, showed that isolates ET001 and ET003 were more aggressive while isolate ET005 was less

aggressive isolate. Both morphology and molecular results showed that, the isolates were identified as

E. turcicum.

Mol Biol 2018, Volume 7

DOI: 10.4172/2168-9547-C1-003