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conferenceseries
.com
Volume 7
Molecular Biology: Open Access
Molecular Biology Meet 2018
August 27-28, 2018
August 27-28, 2018 Dubai, UAE
Molecular Biology and Medicine
International Conference on
Effects of estrogen related receptor α inhibition on metabolic adaptations to endurance training in skeletal muscle
of male wistar rats
Soheil Aminizadeh
Kerman University of Medical Sciences, Iran
F
uel consumption is dependent on severity and duration of the exercise. Medium-Chain Acyl-CoA Dehydrogenase (MCAD)
and Carnitine Palmitoyl Transferase 1 (CPT1) are involved in this process. MCAD performs the first step in fatty acid oxidation
and CPT1 catalyzes the limiting step in mitochondrial beta oxidation. Estrogen-Related Receptor-alpha (ERRα) regulates the
genes involved in fatty acid oxidation and mitochondrial biogenesis. The aim of this study was to investigate the effect of 4 weeks
of endurance training on the expression of genes involved in lipid metabolism. 30 male wistar rats (8 weeks-old) were randomly
divided into four groups: Control (n=7), XCT790 (n=8), trained (n=8) and trained+XCT790 (n=7). Expression of ERRα, MCAD,
CPT-1β, PGC-1α, PDK4 and CS genes was measured by Real-Time PCR and quantified by 2
-∆∆CT
method. The expression of ERRα,
MCAD, CPT1β, PGC-1α, PDK4 and CS genes were significantly higher in trained compared to control group. The expression
of MCAD and CPT1β genes was significantly lower in tained+XCT790 compared trained and the expression of PDK4, CPT1β
and PGC-1α were significantly higher in Trained+XCT790 compared to control+XCT790. Taken together, expression of ERRα
is a trainable factor and its changes are parallel with higher expression of the enzymes which involved in lipid metabolism. This
indirectly suggests a significant role of ERRα in adaptation of lipid metabolism evoked by endurance training.
soheilaminizadeh@yahoo.comMol Biol 2018, Volume 7
DOI: 10.4172/2168-9547-C1-003
Notes: