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Volume 7 Molecular Biology: Open Access Molecular Biology Meet 2018
August 27-28, 2018
August 27-28, 2018 Dubai, UAE
Molecular Biology and Medicine
International Conference on
Nanobiosensor application in detecting biomarkers in Candida infection
Suad Saleem Mubarak, Nadja Melo and Steve Conlan
Swansea University, UK
Candida colonization occurs in the oral cavity, gut and reproductive tract in healthy individuals but is a common cause of fungal
infection in high risk patients (immunocompromised patients, transplants, diabetes, etc.) with prevalence of 50% in intensive
care units. Numerous biomarkers have been anticipated to be of potential use for prognosis in septicemia, including cytokines,
cell-surface markers, acute proteins, coagulation factors and apoptosis factors. Sample size is a critical factor and it is unclear if it
could predict clinical outcome. This proof of concept study investigated biomarkers of Candida immune response from various
body fluid samples using a biosensor method. The successful detection of cytokine IL-17A (infection key marker) and Candida-
antibody were used as biomarkers to detect infection and immune response in saliva, serum, plasma and semen. A specific, label-
free, immunosensor was assembled using polyaniline electropolymerization on a graphene screen-printed electrode base and
antibodies covalent binding against IL-17A and Candida. Limits of detection of 0.250 pg/ml (IL-17A) and 7 Candida cells/ml were
achieved within a linear range of R²=0.98 and R²=0.97, respectively. Sample volume used in the nanosensor method was greatly
reduced in comparison with the traditional methods. Diverse fluid samples from various body sites from the same participants
tested in this study were also detectable. Nanosensors used in the present study were able to detect Candida cells and IL-17A level
in comparison to gold standard traditional methods. Further studies are needed to characterize specificity and sensitivity of the
diagnosis method using nanosensor.
Mol Biol 2018, Volume 7
DOI: 10.4172/2168-9547-C1-003