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conferenceseries.com
Volume 5, Issue 5 (Suppl) Nat Prod Chem Res
ISSN: 2329-6836 NPCR, an open access journal Pharmacognosy 2017
July 24-25, 2017
July 24-25, 2017 Melbourne, Australia
5th International Conference and Exhibition on
Pharmacognosy, Phytochemistry
& Natural Products
Study of phenolic content and anti-microbial activity of Thai sappan wood and pomegranate peel extracts
Subongkoch Subtaeng
Ministry of Science and Technology, Thailand
Thai sappan wood and pomegranate peel were extracted by maceration technique with water and ethanol as a solvent. The total
phenolic content with Folin-Ciocalteau colorimetric method and antimicrobial activity were studied in these extracts. The result
showed that the ethanol extract of pomegranate peel had the highest total phenolic content (73.53 ± 0.04 mg GAE/g) followed by
the water extract of pomegranate peel (70.29 ± 0.04 mg GAE/g), the ethanol extract of sappan wood and the water extract of sappan
wood (61.71 ± 0.02 mg GAE/g and 56.36 ± 0.06 mg GAE/g), respectively. The agar well diffusion method followed with macro-
broth dilution were employed to evaluate the antimicrobial activity against four strains of bacteria (Micrococcus sedentarius DMST
9365, Micrococcus sedentarius DMST 37451, Corynebacterium xerosis DMST 17001, and Propionibacterium acnes DMST 14914)
and one strain of yeast (Candida albicans ATCC 10231). Moreover, the results also presented that both water and ethanol extract of
sappan wood had better antimicrobial activities against all four strains of bacteria than the water extract and the ethanol extract of
pomegranate peel. Meanwhile, the water extract and the ethanol extract of pomegranate peel showed the higher antimicrobial activity
against Candida albicans than the water extract and the ethanol extract of sappan wood.
Biography
Subongkoch Subtaeng is a senior researcher and head of research and development group of Thai herbal products in the Department of Science Service, the
Ministry of Science and Technology, Thailand.
Subongkoch Subtaeng, Nat Prod Chem Res 2017, 5:5 (Suppl)
DOI: 10.4172/2329-6836-C1-017