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.com
Volume 5, Issue 5 (Suppl)
Nat Prod Chem Res
ISSN: 2329-6836 NPCR, an open access journal
Pharmacognosy 2017
July 24-25, 2017
July 24-25, 2017 Melbourne, Australia
5
th
International Conference and Exhibition on
Pharmacognosy, Phytochemistry
& Natural Products
Inhibition of phospholipase A
2
of
Naja nigricollis
by oleanolic acid acetate from
Cryptolepis oblongifolia
Saifullahi Umar
1
, Bashir Zubairu Chedi
1
, Garba Ibrahim
2
, Ahmed Abubakar
2
and Balarabe Abdullahi Sallau
2
1
Bayero University Kano, Nigeria
2
Ahmadu Bello University, Nigeria
C
ryptolepis oblongifolia
(Meisn.) Schltr. is a shrub, traditionally used in treatment of snakebite. Column chromatography of ethyl
acetate extract yielded an isolate which was tested against purified phospholipase A
2
of
Naja nigricollis
venom.The
1
HNMR spectra
of the isolate revealed present of eight quaternary methyl while the
13
CNMR indicate carboxylic acid and carbonyl ester signals, by
comparison with literature the isolate was found to be oleanolic acid acetate which inhibit phospholipase A
2
in a dose dependent
fashion with inhibition binding constant
ki
1.7 µg/ml. The relevance of these finding could serve as bases for the development of
antivenin.
saifullahiumar.su@gmail.comNat Prod Chem Res 2017, 5:5 (Suppl)
DOI: 10.4172/2329-6836-C1-017