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conferenceseries
.com
Volume 5, Issue 5 (Suppl)
Nat Prod Chem Res
ISSN: 2329-6836 NPCR, an open access journal
Pharmacognosy 2017
July 24-25, 2017
July 24-25, 2017 Melbourne, Australia
5
th
International Conference and Exhibition on
Pharmacognosy, Phytochemistry
& Natural Products
Stilbene glycosides and flavonoids from the roots of
Euphorbia armena
L N Gvazava
TSMU Iovel Kutateladze Institute Pharmacochemistry, Georgia
P
lants of the genus Euphorbia are widely distributed around the whole world. About 45 of the more than 800 known species
are indigenous to Georgia. The chemical composition of many plants of this genus has been well studied. We have previously
studied the chemical compositions of
E. armena
Prokh., and
E. glareosa
Pall. ex- Bieb., plants of the Georgian flora. We isolated
and characterized the new hydrolyzed tannins glareins A, B and C. In continuation of the study of the chemical composition of
E. armena
, we isolated total phenolic compounds, which contained stilbenes from the plant roots. Raw material was extracted
three times by refluxing in MeOH (80%) for 1 hour, and then solvent was removed. The remaining aqueous phase was treated with
hexane to remove lipophilic substances and then extracted with EtOAc. The EtOAc fraction was chromatographed over a column of
Sephadex LH-20 with gradient elution by H
2
O:MeOH with an increasing alcohol concentration to produce six fractions. Fractions
5 and 6 contained stilbenes and were combined and re-chromato¬graphed over an analogous column with elution by EtOH to
isolate three pure compounds. Compound 1 was identified as 2-O-β -D-glucopyranosyl-2, 3, 5, 4'-tetrahydroxystilbene. Compound
2 was identified as 2-O-[β -D-glucopyranosyl-(2"-O-galloyl)]-2,3,5,4'-tetrahydroxystilbene. Compound 3 was identified as 2-O-[β
-D-glucopyranosyl-(3"-O-galloyl)]-2,3,5,4'-tetra¬hydroxystilbene. All studied stilbene glycosides were isolated and described for the
first time from plants of the genus Euphorbia. From the EtOAc fraction were isolated three derivatives of kaempferol-kaempferol-
(3,5,7,4'-tetrahydroxyflavone), kaempferol-3-O-β-D-glucopyranoside (astragalin) and kaempferol-3-O-α-D-arabinopyranoside. All
studied flavonoids were isolated for the first time from the title plants.
Biography
Lili Gvazava is the Professor of Pharmacy at the Tbilisi State Medical Universitu’s Iovel Kutateladze Institute of Pharmacochemistry, Tbilisi, Georgia. She has
various publications in national and International Journals.
liligvazava@yahoo.comL N Gvazava, Nat Prod Chem Res 2017, 5:5 (Suppl)
DOI: 10.4172/2329-6836-C1-017