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conferenceseries.com
Volume 5, Issue 5 (Suppl) Nat Prod Chem Res
ISSN: 2329-6836 NPCR, an open access journal Pharmacognosy 2017
July 24-25, 2017
July 24-25, 2017 Melbourne, Australia
5th International Conference and Exhibition on
Pharmacognosy, Phytochemistry
& Natural Products
Blue, green and UV lights inhibit Scrophularia yoshimura hairy roots growth but enhance their secondary
metabolites production
Yi-Chen Liu1, Chi-Chen Lin1 , Wen-Te Chang2, Meng-Shiou Lee2 and Ming-Kuem Lin2
1National Chung-Hsing University, Taiwan
2China Medical University, Taiwan
Scrophularia yoshimura (Scrophulariaceae) is a Taiwan endemic plant. It has been proved that its main active compounds are higher
than Scrophularia ningpoensis (Chinese figwort, xuán cān). Transformed hairy root which grows rapidly is used to produce large
quantity of active compounds for many medicinal plants. However, there is no research about different wavelengths of light influence
transformed hairy root. The aim of this study is to investigate the effect of different wavelengths of light on S. yoshimura hairy
root. In this study, S. yoshimura hairy roots were inducted by Agrobacterium rhizogenes LBA1334. Different combinations of various
wavelength light-emitting diodes (LEDs) were used to irritate S. yoshimura hairy root for 4 weeks. The content of harpagoside and
cinnamic acid in the hairy root were analyzed by high performance liquid chromatography. We found that S. yoshimura hairy root
irritated by red light and infrared light increase both fresh and dry weight, but reduce the amounts of harpagoside and cinnamic acid
compared to dark-culture. On the other hand, S. yoshimurae hairy root irritated by blue light, green light and UV light reduce both
fresh and dry weight, but increase the amounts of harpagoside and cinnamic acid. The results of this study is the first time showed
that different wavelengths of light affect transformed hairy root growth and secondary metabolites production of S. yoshimura. These
findings can be applied in the large production of harpagoside and cinnamic acid.
Biography
Yi-Chen Liu is currently a PhD student at National Chung-Hsing University, Taiwan.
Yi-Chen Liu et al., Nat Prod Chem Res 2017, 5:5 (Suppl)
DOI: 10.4172/2329-6836-C1-017