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conferenceseries.com
Volume 8, Issue 5 (Suppl) J Chromatogr Sep Tech, an open access journal
ISSN: 2157-7064 Chromatography 2017
August 07-09, 2017
August 07-09, 2017 | Rome, Italy
4th World Congress on
Chromatography
Platform chromatographic purification of AAV gene therapy vectors
Aleš Štrancar and R Sekirnik
BIA Separations, Slovenia
Adeno-associatedvirus (AAV) vectors of various serotypes are considered tohavehighpotential for gene therapy applications.
Currently, manufacturing of AAV vectors faces the challenge of co-production of incompletely formed particles lacking
a recombinant viral genome. Empty capsids increase the dose of total AAV administered for efficient transduction and are
thought to cause unwanted immunological reactions against the virus. Removal of empty capsids during manufacturing,
as well as analysis of empty/full AAV particle content is therefore a critical requirement for any AAV production process.
Poster demonstrates how CIMmultus™ QA monolithic columns can be used to remove empty AAV capsids from the product
chromatographically in a single step.
Image 1: CIMmultusTM QA column strong ion exchange chromatographic separation of empty and full capsids.
Biography
Aleš Štrancar is the CBDO of the BIA Separations and one of the main inventors of the CIM Convective Interaction Media®. He is author and co-author of more
than 60 scientific papers dealing with separation and purification technologies, a co-author of five granted USA patents and their foreign equivalents in the field of
biomolecule separations and purification and a co-author of several book chapters dealing with novel chromatography technologies for biomolecule separation. He
co-developed several industrial scale purification processes. He was the President of Technology Council of Ministry of Economy of the Republic of Slovenia and
Member of Science and Technology Council of the Republic of Slovenia.
Aleš Štrancar et al., J Chromatogr Sep Tech 2017, 8:5(Suppl)
DOI: 10.4172/2157-7064-C1-032