4 / 35
Page 55
Notes:
conferenceseries
.com
Volume 8, Issue 5 (Suppl)
J Chromatogr Sep Tech, an open access journal
ISSN: 2157-7064
Chromatography 2017
August 07-09, 2017
August 07-09, 2017 | Rome, Italy
4
th
World Congress on
Chromatography
HPLC-fluorescence method for the enantioselective analysis of propranolol in rat serum using
immobilized polysaccharide-based chiral stationary phase
Aymen K Al-Suwailem
1
, Amer M Alanazi
1
, Mohamed M Hefnawy
1
, Abdulrahman A Al-Majed
1
, Mohamed G Kassem
1
, Gamal A Mostafa
1
, Sabry M
Attia
1
and
Mohammed M Khedr
2
1
King Saud University, Saudi Arabia
2
Cardiff University, UK
A
stereoselective high-performance liquid chromatographic (HPLC)methodwas developed and validated todetermine S-(-)-
andR-(+)-propranolol in rat serum. Enantiomeric resolutionwas achieved on cellulose tris(3, 5-dimethylphenylcarbamate)
immobilized onto spherical porous silica chiral stationary phase (CSP) known as chiralpak IB. A simple analytical method
was validated using a mobile phase consisted of n-hexane-ethanol-triethylamine (95:5:0.4%, v/v/v) at a flow rate of 0.6 mL
min-1 and fluorescence detection set at excitation/emission wavelengths 290/375 nm. The calibration curves were linear over
the range of 10–400 ng mL
-1
(R=0.999) for each enantiomer with a detection limit of 3 ng mL
-1
. The proposed method was
validated in compliance with ICH guidelines in terms of linearity, accuracy, precision, limits of detection and quantitation,
and other aspects of analytical validation. Actual quantification could be made for propranolol isomers in serum obtained
from rats that had been intraperitoneally (i.p.) administered a single dose of the drug. The proposed method established in
this study is simple and sensitive enough to be adopted in the fields of clinical and forensic toxicology. Molecular modeling
studies including energy minimization and docking studies were first performed to illustrate the mechanism by which the
active enantiomer binds to the β-adrenergic receptor and second to find a suitable interpretation of how both enantiomers
are interacting with cellulose tris(3,5-dimethylphenylcarbamate) CSP during the process of resolution. The latter interaction
was demonstrated by calculating the binding affinities and interaction distances between propranolol enantiomers and chiral
selector.
Biography
Aymen K Al-Suwailem is working as an Assistant of Program Director at Prince Sultan Cardiac Center, KSA. He has completed PhD and Master’s degree in
Analytical Pharmaceutical Chemistry. He has also completed his Master’s degree in Clinical Pharmacology and Hospital Administration.
aymen_4120@hotmail.comAymen K Al-Suwailem et al., J Chromatogr Sep Tech 2017, 8:5(Suppl)
DOI: 10.4172/2157-7064-C1-032