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conferenceseries
.com
Volume 12
Journal of Molecular and Genetic Medicine
ISSN: 1747-0862
May 21-23, 2018 Barcelona, Spain
&
Integrative Biology
6
th
International Conference on
Genomics and Molecular Biology
10
th
International Conference on
Genomics 2018 and Integrative Biology 2018
May 21-23, 2018
JOINT EVENT
Expression of alpha 1 intensities in haptoglobin 2-1 and its association with clinical course in
aneurysmal subarachnoid hemorrhage
Bong Jun Kim
1
, Young Mi Kim
1
and
Jin Pyeong Jeon
1, 2
1
Institute of New Frontier Research - Hallym University College of Medicine, Republic of South Korea
2
Hallym University College of Medicine, Republic of South Korea
Introduction&Aim:
Delayed cerebral ischemia (DCI) contribute to poor clinical outcome following subarachnoid hemorrhage
(SAH). Haptoglobin (Hp) comprised of two light (α) and two heavy (β) chains has anti-oxidant effect by free hemoglobin (Hb)
binding. Among three phenotypes, Hp1-1 (two α1), Hp2-1 (α1 and α2), and Hp2-2 (two α2), higher protective effect for toxic
free Hb is reported in Hp2-2 than Hp1-1. However, few studies have focused on Hp2-1 in determining outcome. This study
aims to examine the α1 and α2 expression and to evaluate the association with outcomes in Hp2-1.
Methodology:
Eighty-seven patients were prospectively enrolled: Hp1-1 (12, 13.8%); Hp2-1 (36, 41.4%); and Hp2-2 (n=39,
44.8%). Phenotypes was confirmed by western blotting. The relative intensities were measured as α intensities divided by
the albumin intensities and expressed as the median (25
th
-75
th
percentile). The difference in α intensities according to DCI,
angiographic vasospasm (AV) and outcome (mRS 0-2) in 6 months were analyzed.
Results:
DCI (n=21, 53.8%) and AV (n=22, 56.4%) were more frequently observed in Hp2-2 than Hp1-1 (DCI, n=3 (25.0%)
and AV, n=3 (25.0%)). The α1 intensities in Hp2-1 without DCI (0.70 (0.54-0.89)) and AV (0.65 (0.32-0.88)) were significantly
higher than that with DCI (0.24 (0.14-0.32), p<0.001) and AV (0.32 (0.17-0.67), p=0.046). For α2 intensities, no significant
difference was noted according to DCI (p=0.377) and AV (p=0.459). The α1 (p=0.359) and α2 (p=0.233) intensities did not
differ significantly according to outcome.
Conclusions:
Higher α1 intensities in Hp2-1 can be associated with lower DCI and AV. The α1 intensity degree may provide
additional information on individual risk of secondary injury following SAH in Hp2-1.
Biography
Bong Jun Kim completed his Graduation from Hallym University, Department of Biomedical Sciences and studied experimentation in the virology laboratory for
about a year during his undergraduate degree. In the same year, he acquired a certificate for handling experimental animals. He majored in Medical Genetics with
a Master's degree. He studied COPD-associated gene mutations in a Korean cohort through next-generation genome analysis and statistical analysis. Currently,
he collaborates with Neurosurgeons as a member of industry-academia cooperation group and studying neurosurgical diseases.
luckykbj@naver.comBong Jun Kim et al., J Mol Genet Med 2018, Volume 12
DOI: 10.4172/1747-0862-C2-028