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conferenceseries
.com
Volume 12
Journal of Molecular and Genetic Medicine
ISSN: 1747-0862
May 21-23, 2018 Barcelona, Spain
&
Integrative Biology
6
th
International Conference on
Genomics and Molecular Biology
10
th
International Conference on
Genomics 2018 and Integrative Biology 2018
May 21-23, 2018
JOINT EVENT
Comparison of the effect of caloric restriction types on the expression of some cancer-associated
genes via RNA-seq
Nehir Ozdemır Ozgenturk
1
, Ali Yasir Koç
1
, Zehra Omeroglu Ulu
1
, Soner Dogan
2
, Bilge Guvenc Tuna
2
and
Salih Ulu
1
1
Yildiz Technical University, Davutpasa Campus, Turkey
2
Yeditepe University, Turkey
P
athways and Co-expression Network Analysis of Immune-Related Genes in Short-term Calorie Restricted Mice RNA-seq
technology was performed a comparative transcriptome analysis of the MMTV-TGF-α female mice thymus tissues that
were fed
ad libitum
(AL), cronic calorie restriction (CCR) (85% of AL fed mice) and intermittent calorie restriction (ICR) (3
weeks AL fed, 1 week 40% of AL fed mice) from 10 weeks of age to 17 weeks of age or 18 weeks of age. The results of
RNA-seq analysis, a total of 6091 significantly differentially expressed genes (DEGs) were identified. 2821, 2825 and 445
significantly DEGs were detected between AL-CCR, CCR-ICR and AL-ICR fed groups, respectively. These DEGs
were classified according to cellular components, biological processes and molecular functions Gene Ontology (GO) main
categories. 188 of 2821, 36 of 445, 176 of 2825 genes were identified to be involved in immune system process (GO:0002376)
biological processes GO categories. KEGG pathway and the gene co-expression network analysis between AL-CCR, CCR-ICR
and AL-ICR fed groups immune-related DEGs were done using String database. For network analysis, nodes
and edges were presented the interaction between immune-related DEGs.Calorie restriction is to reduce the amount
of calorie received without malnutrition. This manipulation method has positive effects on life span, cancer formation and
immune response in the long run. However, there are also some systems such as lymph organs that respond quickly to lack of
calories. Thymus is one of these lymph organs. In this study, two type calorie restriction practices (CCR/ICR) were used on
MMTV-TGF- α transgenic mice and a group of mice were fed
ad libitum
(AL) as a control group. It is aimed to determine the
changes that the nutrition type will bring about in the expression of genes. The manipulation started at the 10th week of the life
of the mice and ended at the 17th and 18th week. RNA-Seq-based transcriptome were performed to the RNAs obtained from
the thymus of the sacrificed mice. Through RNA-Seq results “Differential Expressed Genes” (DEGs) were determined. 6091
of them have been identified as statistically significant (p <0.05). The AKT1, CTCF, PTEN genes known to cause breast tumor
development were selected by data banks. The selected genes were searched in three different RNA-Seq data and expression
levels were determined for three genes in three different medium. Changes in expression level are displayed via graphics .
Biography
Nehir Ozdemır Ozgenturk completed her Graduation at Ege University; Master's Degree in Plant Breeding Department and PhD in the Department of Justus
Liebieg University. In 2003, she worked at the Cereal Research Center in Canada as a Post-doc. Also she worked at Georgia Medical School for four mounths with
Nato fellowship. She has scientific paper in various scientific journals, publications and presentations at international conferences.
nehirozdemir@yahoo.comNehir Ozdemır Ozgenturk et al., J Mol Genet Med 2018, Volume 12
DOI: 10.4172/1747-0862-C2-028