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Volume 12 Journal of Molecular and Genetic Medicine
ISSN: 1747-0862
May 21-23, 2018 Barcelona, Spain
&
Integrative Biology
6th International Conference on
Genomics and Molecular Biology
10th International Conference on
Genomics 2018 and Integrative Biology 2018
May 21-23, 2018
JOINT EVENT
Comparative genomics analysis of 29 Lactococcus lactis strains
Schermann Sabine
DuPont Nutrition and Health, France
Lactococcus lactis is a lactic acid bacterium widely used in the dairy industry to produce diverse cheeses. Several decades of
meticulous microbial selection have provided large collections of strains with appropriate technological attributes such as
fast milk acidification, improved bacteriophage resistance and desired aroma production. The objective of this study is to link
specific phenotypes to the genetic content of select strains using a pan-genome approach following whole genome sequencing.
Whole genome sequences were generated for 29 L. lactis subsp. cremoris or subsp. lactis proprietary strains using Illumina
sequencing. The 29 draft genomes ranged in size between 2.40 and 2.90 Mb (mean: 2.57 Mb) and were organized into 94 to 332
contigs, reflecting a varying content of repeated sequences, notably insertion sequences. The number of predicted CDS varied
between 2,644 and 3,521 per genome (mean: 2,813). In most genomes, putative plasmid-based contigs could be detected,
although this prediction of plasmid nature is not trivial. Overall, 81,578 CDS were classified into 10,604 gene families (pan-
genome), including 1,142 core genes and 4,769 unique genes. In this study, many novel genes and functions could be identified
easily within a set of 29 L. lactis strains having a potential for industrialization. Although this species is well-known for its small
genome size, our data indicate a significant strain-to-strain genetic diversity in agreement with already observed physiological
distinctive features thus paving the way for further genomic analyses.
Biography
Schermann Sabine obtained her Master’s Degree in Bioinformatics at Université Paul Sabatier (Toulouse, France) in 2011. Just after her studies, she joined the
DuPont Company as a Bioinformatician. She works in the Research and Development Department-Nutrition and Health division of the same company. Her team
works on the selection and study of lactic acid bacteria aimed to be used in the dairy industry. Her main missions are tool development, Linux server maintenance
and the conduction of genomics analyses.
Schermann Sabine, J Mol Genet Med 2018, Volume 12
DOI: 10.4172/1747-0862-C2-028