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Volume 12 Journal of Molecular and Genetic Medicine
ISSN: 1747-0862
May 21-23, 2018 Barcelona, Spain
&
Integrative Biology
6th International Conference on
Genomics and Molecular Biology
10th International Conference on
Genomics 2018 and Integrative Biology 2018
May 21-23, 2018
JOINT EVENT
APtDRG1, desiccation response gene from Pyropia tenera (Rhodophyta) exhibits chaperone function
and enhance abiotic stress tolerance
Dong Woog Choi
Chonnam National University, South Korea
Pyropia are commercially valuable marine red algae that grow in the intertidal zone and extremely tolerant to desiccation
stress. We identified and reported the desiccation response genes (DRGs) based on comparison of the transcriptomes of P.
tenera. Among them, PtDRG1 encodes a polypeptide of 22.6 kDa that located in chloroplast. PtDRG1 does not share sequence
homology with known genes in public database except for several red algae species. Transcription of the PtDRG1 gene was
upregulated by osmotic stress induced by mannitol or H2O2 as well as desiccation stress but did not respond to heat. When
PtDRG1 was over-expressed in Escherichia coli and Chlamydomonas, the transformed cells grew much better than control cells
under high temperature as well as osmotic stress induced by mannitol and NaCl. In addition, PtDRG1 significantly reduced
the thermal aggregation of substrate protein at heat stress condition. These results demonstrate that PtDRG1 have a chaperone
function and plays a role in tolerance mechanism for abiotic stress in Pyropia. This study shows that red algae have unknown
stress proteins such as PtDRG1, and that these proteins have chaperon function and play a role in stress tolerance in red algae
as stress proteins such as dehydrin work in green plants.
Biography
Dong Woog Choi has completed his PhD from Seoul National University, South Korea and Postdoctoral studies from University of California at Riverside, USA. He
is the Professor in the Department of Biology Education, Chonnam National University, South Korea.
Dong Woog Choi, J Mol Genet Med 2018, Volume 12
DOI: 10.4172/1747-0862-C2-028