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.com
Volume 12
Journal of Molecular and Genetic Medicine
ISSN: 1747-0862
May 21-23, 2018 Barcelona, Spain
&
Integrative Biology
6
th
International Conference on
Genomics and Molecular Biology
10
th
International Conference on
Genomics 2018 and Integrative Biology 2018
May 21-23, 2018
JOINT EVENT
Mussel adhesive protein-conjugated vitronectin (MAP151-V) induces anti-inflammatory activity on
LPS-stimulated macrophages and UVB-irradiated keratinocyte
Kyung Bae Pi
1
, Seul Gee Um
1
, Jung Mo Ahn
1
, Beom Seop Rho
1
, Ki Beom Lee
1
, Sung Gil Park
2
, Ho Jin Kim
1
and
Yoonjin Lee
3
1
Biotechnology & Business Center, Incheon business information Technopark, Incheon, Korea
2
R&D center, Advanced BioTech Co., Ltd, Pilot Plant 12 Gaetbeol-ro, Yeonsu-gu, Incheon, Korea
3
Cosmocos Corporation, Incheon, Korea
S
kin inflammation and dermal injuries is a major clinical problem due to the current therapies limited to established scars
with poor understanding of healing mechanisms. Unique adhesive and biocompatibility properties of mussel adhesive
proteins (MAPs) are known for their great potential in many tissue engineering and biomedical applications. Previously it was
successfully demonstrated that redesigned hybrid type MAP, fb-151, mass produced in gram-negative bacterium Escherichia
coli, could be utilized as a promising adhesive biomaterial. However, the biological activity of vitronectin-bound recombinant
fb-151 has not been established.The aimof this studywas to develop a novel recombinant protein usingMAP and vitronectin and
to elucidate the anti-inflammatory effects of these on macrophages and keratinocytes. We investigated the anti-inflammatory
activities of recombinant fb-151 conjugated vitronectin (MAP151-V). LPS (Lipopolysaccharide) was used as a stimulant for
macrophages and UVB was used as a stimulant for keratinocytes. Macrophages stimulated by LPS increased the expression of
iNOS and COX-2, which are inflammatory factors, while the MAP151-V -treated groups suppressed the expression of iNOS
and COX-2 in a dose-dependent manner. In addition, keratinocyte stimulated with UVB showed reduced expression of iNOS
and COX-2 MAP151-V treatment. Interestingly, in UVB-irradiated keratinocytes, inflammatory cytokines such as IL-1
β
, IL-6
and TNF-αwere significantly reduced by MAP151-V treatment. These results suggest that MAP151-V has a more effective
anti-inflammatory activity on keratinocyte, suggesting its use as a skin inflammation and therapeutic agent of skin..
Biography
Kyung Bae Pi completed his MS from Kyung Hee University, South Korea. He is a Senior Researcher and Project Leader of Incheon Business Information
Technopark, South Korea. He has published more than 14 papers in reputed journals and has been serving as an Editorial Board Member of repute.
kbpi@ibitp.or.krKyung Bae Pi et al., J Mol Genet Med 2018, Volume 12
DOI: 10.4172/1747-0862-C2-028