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conferenceseries.com
Volume 5, Issue 5 (Suppl) Nat Prod Chem Res
ISSN: 2329-6836 NPCR, an open access journal Pharmacognosy 2017
July 24-25, 2017
July 24-25, 2017 Melbourne, Australia
5th International Conference and Exhibition on
Pharmacognosy, Phytochemistry
& Natural Products
Search for natural compounds that promote CHOP-induced apoptosis
Young Sook Yun1, Yukari Takeda1 , Hiroyuki Fuchino2, Nobuo Kawahara2, Shigeru Takahashi1, Yuji Takahashi1 and Hideshi Inoue1
1Tokyo University of Pharmacy and Life Sciences, Japan
2Research Center for Medicinal Plant Resource, Japan
The transcription factor CHOP, also known as GADD 153, increases expression level and induces apoptosis in response to
endoplasmic reticulum stress caused by various factors including genetic mutation, pathogens and active oxygen. In order to
search for natural compounds that induce CHOP expression, we screened about 6,000 kinds of methanol extracts from various higher
plants and their different parts using a luciferase reporter gene assay with CHOP promoter in Mia PaCA-2 cells. In the first screening,
121 extracts showed induction of 2-fold or more luciferse activity compared to the control. By a second screening on the 121 extracts,
6 extracts showing 3-fold or more luciferase inducing activity were selected. Among them, the extract of Macleaya cordata (Willd.)
was separated on silica gel column chromatography and then HPLC was done. Two compounds were thereby isolated, then identified
as dihydrosanguinarine and 6-methoxydihydrosanginarine based on NMR and MS spectral data. Both compounds were found to
induce CHOP promoter-driven luciferase expression in the reporter gene assay, but did not significantly affect endogenous CHOP
protein levels. Dihydrosanguinarine increased the levels of cleaved-PARP and cleaved-caspase-3 known as apoptosis markers. This
suggests that dihydrosanguinarine may induce apoptosis not only through the ER stress pathway but also through other pathways.
Although sanguinarine was reported to be a major compound of M. cordata, it was not isolated in this study. This may have been due
to conversion of sanguainarine to 6-methoxysanguinarine during extraction with MeOH.
Biography
Young Sook Yun has completed her PhD from Tokyo University of Pharmacy and Life Sciences and Postdoctoral studies from Korea Institute Biosciences
and Biotechnology and National Institute of Health Science in Japan. Recently, her researches have been focused on natural chemistry related to cancer and
neurosciences.
Young Sook Yun et al., Nat Prod Chem Res 2017, 5:5 (Suppl)
DOI: 10.4172/2329-6836-C1-017