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.com
Volume 5, Issue 5 (Suppl)
Nat Prod Chem Res
ISSN: 2329-6836 NPCR, an open access journal
Pharmacognosy 2017
July 24-25, 2017
July 24-25, 2017 Melbourne, Australia
5
th
International Conference and Exhibition on
Pharmacognosy, Phytochemistry
& Natural Products
Antibacterial, anti-mycobacterium and cytotoxic activities of Tin fruit (
Ficus carica
) compounds in Java
Cikra Ikhda
1
, Andri Priyoherianto
1
, Fahmi Ardianti
1
, Elly Purwati
1
, Malikul Habsy
1
and Joyce
2
1
Academy of Pharmacy Mitra Sehat Mandiri, Indonesia
2
Universiteit Leiden, Netherland
T
in Fruit (
Ficus carica
) is one of famous fruit in Indonesia. The antibacterial and cytotoxicity test of pure compounds of
Ficus
carica
were done, which was collected from Sidoarjo, East Java, Indonesia. Isolation and purification of the crude extracts and
the pure compounds were carried out using several chromatographic techniques. The structures of the isolated compounds were
elucidated by spectroscopic methods such as UV, IR, 1D (
1
H,
13
C, DEPT) and 2D (COSY, HMQC and HMBC), NMR and MS. Four
compounds were identified such as β-sitosterol, 6-(2-methoxy-Z-vinyl)-7-methyl-pyranocoumarin and 9,19-cycloartane triterpenoid
were isolated from this plant. These compounds were screened for their antibacterial and cytotoxic activities. Significant antibacterial
activities were shown by compounds against
Staphylococcus aureus
and
Bacillus subtilis
and also for
Mycobacterium tuberculosis
and
Mycobacterium marinum
in high concentration. Meanwhile, the screening for cytotoxicity using SRB assay on MCF-7, SKOV3, HT-
29 and MDA-MB-231 cell lines for these compounds revealed the percentage of cells survival at doses 15 μg/ml were higher than 50%.
cikraikhda@gmail.comNat Prod Chem Res 2017, 5:5 (Suppl)
DOI: 10.4172/2329-6836-C1-017