30 / 41
Page 74
conferenceseries
.com
Volume 5, Issue 5 (Suppl)
Nat Prod Chem Res
ISSN: 2329-6836 NPCR, an open access journal
Pharmacognosy 2017
July 24-25, 2017
July 24-25, 2017 Melbourne, Australia
5
th
International Conference and Exhibition on
Pharmacognosy, Phytochemistry
& Natural Products
Development and validation of a rapid LC-MS/MS method for simultaneous determination of Kaempferol
and Quercetin in
Thespesia populnea
extract
Hiteksha S Panchal
1
and Mamta B Shah
2
1
Anand Pharmacy College, India
2
LM College of Pharmacy, India
I
n this study, a simple and rapid liquid chromatography-tandem mass spectrometry (LC-MS/MS) method was developed and
validated for simultaneous determination of Kaempferol and Quercetin in
Thespesia populnea
extract. The compounds were eluted
using Gemini C18, (50×2.0 mm, 3μm) with the mobile phase consisting of acetonitrile and 0.3% formic acid in water at the flow rate
of 0.400 mL/min. The assay exhibited a linear dynamic range of 25-2500 µg/ml for Kaempferol and Quercetin. The values for both
intraday and interday precision and accuracy were within the generally accepted criteria for analytical methods (<15%). Selectivity,
linearity, limit of detection (LOD), limit of quantification (LOQ), accuracy and precision were evaluated for all analytes. The proposed
method is more accurate and sensitive can be used for the routine quantification of the Kaempferol and Quercetin in the herbal
extracts as well as polyherbal formulations.
hitekshapanchal22@yahoo.comNat Prod Chem Res 2017, 5:5 (Suppl)
DOI: 10.4172/2329-6836-C1-017