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.com
Volume 5, Issue 5 (Suppl)
Nat Prod Chem Res
ISSN: 2329-6836 NPCR, an open access journal
Pharmacognosy 2017
July 24-25, 2017
July 24-25, 2017 Melbourne, Australia
5
th
International Conference and Exhibition on
Pharmacognosy, Phytochemistry
& Natural Products
Evidence based validation of
Dendrobium fimbriatum
as a hepatoprotectant: An orchid from Northeast
India
Sunita Shailajan, Suhas Pednekar, Sasikumar Menon and Deepti Gurjar
Ramnarain Ruia College, India
D
endrobium fimbriatum
Hooker., is an important orchid found in Northeast India. It is used by the locals as traditional medicine
in resetting of fractured bones and possesses antioxidant activity. It has been reported to possess constituents like rhein,
fimbriatone, etc. However, there is a dearth of scientific data on its phytochemical and pharmacological evaluation. Thus, on the basis
of published reports, the present study was aimed to evaluate the hepatoprotective activity of hydroalcoholic extract of
Dendrobium
fimbriatum
stem in CCl4 intoxicated albino Wistar rats. The quality of the plant material was assessed and individual plant parts
were evaluated for the presence of active markers; ursolic acid, β-sitosterol and lupeol using a validated HPTLC and HPLC method.
Safety of the plant extract was established in albino Swiss mice following the OECD guidelines no. 420 and it was found to be safe up
to an oral dose of 2000 mg/kg body weight. Hepatoprotective activity of the extract of
Dendrobium fimbriatum
stem was studied at
three doses in terms of various biochemical parameters and histopathology. The results were compared with a known herbal drug,
Silymarin. Oral administration of the extract of
Dendrobium fimbriatum
stem showed hepatoprotective activity in a dose-dependent
manner and the results were at par with the positive control Silymarin. The results of histopathological observation were also found
to be in compliance with the findings of biochemical parameters analysis suggesting the possible use of
Dendrobium fimbriatum
as
hepatoprotective agent and forms baseline for future pharmacological investigations.
sunitashailajan@gmail.comNat Prod Chem Res 2017, 5:5 (Suppl)
DOI: 10.4172/2329-6836-C1-017