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.com
Volume 5, Issue 5 (Suppl)
Nat Prod Chem Res
ISSN: 2329-6836 NPCR, an open access journal
Pharmacognosy 2017
July 24-25, 2017
July 24-25, 2017 Melbourne, Australia
5
th
International Conference and Exhibition on
Pharmacognosy, Phytochemistry
& Natural Products
Antioxidant potential of
Sapium ellipticum
(Hochst.) Pax. leaf extract against CCL
4
-induced reactive
species
in vivo
Ighodaro O M
1,2
, Akinloye O A
1
, Ugbaja R N
1
and Omotainse S O
3
1
Federal University of Agriculture, Nigeria
2
Lead City University, Nigeria
3
College of Veterinary Medicine, Nigeria
T
he antioxidant potential of
Sapium ellipticum
(SE) leaf extract against CCl
4
-induced reactive species
in vivo
was examined in
adult female Wister rats. Toxicity was induced in the animals via a single intraperitoneal (i.p) dose of CCl
4
(20% 2 mL/Kg of body
weight, BW). SE extract was orally administered twice daily for 28 days at 8 hours interval (400 and 800 mg/kg BW) to different groups
of CCl
4
-treated animals. Its effects were measured against known antioxidants, Butylated hydroxytoluene (BHT) and L-Ascorbic acid
(L-AA). The activities of superoxide dismutase (SOD), catalase (CAT), glutathione-S-transferase (GST) and glutathione peroxidase
(GP
X
) were analyzed in the post mitochondrial fractions (PFM) of the liver and kidney of rats. The level of tissue protein, reduced
glutathione (GSH) and malondialdehyde (MDA) was also estimated. The data obtained showed that SE elicited its antioxidant
functionality mainly through anti-peroxidation effect and promotion of superoxide dismutase and catalase activities. The extract
significantly (p˂0.05) lowered the degree of peroxidation (76.7%) and improved the activities of superoxide dismutase (51.2%) and
catalase (43.5%) relative to the CCl
4
-untreated group. However, its ability to improve endogenous GSH level as well as GST and GP
x
activities was poor. Overall, SE leaf extract appears to have the phyto-proficiency to protect against membrane peroxidation and to
improve the functions of some first line antioxidant enzymes
in vivo
in the face of overwhelming reactive species. This postulation is
substantiated by the identification of antioxidant compounds like α-tocopherol, amentoflavone, lupeol and luteolin-7-O-glucoside in
the active fractions of SE through HPLC-MS technique.
ighodaro.macdonald@lcu.edu.orgNat Prod Chem Res 2017, 5:5 (Suppl)
DOI: 10.4172/2329-6836-C1-017