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Volume 5, Issue 5 (Suppl)
Nat Prod Chem Res
ISSN: 2329-6836 NPCR, an open access journal
Pharmacognosy 2017
July 24-25, 2017
July 24-25, 2017 Melbourne, Australia
5
th
International Conference and Exhibition on
Pharmacognosy, Phytochemistry
& Natural Products
Cytotoxic and antiproliferative evaluations of a novel diterpenoid from
Euphorbia graminea
Ikpefan Emmanuel Oise
Delta State University, Nigeria
B
ioassay guided fractionation of the methanol extract of
Euphorbia graminea
against breast cancer (MCF-7), lung cancer
(NCI-H460) and NIH 3T3 (mouse embryonic fibroblast normal cell line) at 1-250 µg/mL was carried out. Extracts of
E. graminea
was partitioned into aqueous and chloroform fractions and both fractions were tested for their effects on MCF-7 and NCI-H460.
Further chromatographic and biological studies of the active chloroform fraction yielded a compound whose identity was revealed as
Abietane-11, 23 diene-16-oic-14-ones through NMR and MS studies. This compound was observed to give –3.3±1.4 and 5.30±3.75%
cytotoxicity against MCF-7 and NCI-H460 at 100 µMwith GI
50
and TGI of +38±0.74, 96.94±6.95 µM and 53.70±9.30 and 93.88±11.70
µM respectively. The result has established the rationale for the use in ethnomedicinal practice.
ikpefanemmanuel@delsu.edu.ngNat Prod Chem Res 2017, 5:5 (Suppl)
DOI: 10.4172/2329-6836-C1-017