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conferenceseries
.com
Volume 5, Issue 5 (Suppl)
Nat Prod Chem Res
ISSN: 2329-6836 NPCR, an open access journal
Pharmacognosy 2017
July 24-25, 2017
July 24-25, 2017 Melbourne, Australia
5
th
International Conference and Exhibition on
Pharmacognosy, Phytochemistry
& Natural Products
Synthesis and
in vitro
antiplasmodial,
in vitro
antimycobacterial and
in vitro
cytotoxicity studies of oleanolic
acid and its ester
Victor O Fadipe
1
, Abayomi Oguntunde
1
, Hussaini Doko Ibrahim
2
and Andrew R Opoku
3
1
Federal Ministry of Science and Technology, Nigeria
2
Raw Materials Research and Development Council, Nigeria
3
University of Zululand, South Africa
T
uberculosis (TB) and malaria are chronic fatal diseases and attained a dangerous status worldwide. New drugs are therefore
needed to halt the mortality rate. Pentacyclic triterpenes, a group of naturally occurring compound have been reported to have
broad spectrum of biological activities that could be harnessed for development of new drugs for TB and malaria. In a quest to find
new antimalarial and anti-mycobacterial drugs, oleanolic acid (OA) was isolated from the flower buds of
Syzygium aromaticum
.
The derivative, 3-O-Acetyl-Oleanolic acid (OAA), was synthesized from the isolated product and their biological activities were
carried out and compared. The antiplasmodial and antimycobacterial activity of oleanolic acid and its derivative were subsequently
investigated against
Plasmodium falciparum
(Chloroquine Sensitive Strain) NF54 and
Mycobacterium tuberculosis
H
37
RV, respectively.
The compounds were evaluated for cytotoxicity activity using MTT (human embryonic kidney (HEK293) and human liver model
(HepG2)). OAA exhibited IC
50
of 4.3 µg/ml against
P. falciparum
while OA exhibited IC
50
of 27.4 µg/ml. OAA exhibited MIC
99
of
79.8 µg/ml against
M. tuberculosis
while OA exhibited MIC
99
of 73.1 µg/ml. The MTT test (HEK293 and HepG2) were in the range of
IC
50
≥300 μg/ml, indicating low toxicity level. The data obtained above, indicate that both compounds can serves as template for the
synthesis of potent anti-TB and anti-malaria drugs.
vobfadipe@yahoo.comNat Prod Chem Res 2017, 5:5 (Suppl)
DOI: 10.4172/2329-6836-C1-017