Quality Protein

"Heat alone was insufficient to recover high quality protein extracts from FFPE tissue. Elevated pressure promotes water penetration into the inner core of proteins, causing denaturation, whereas heat alone causes protein unfolding followed by aggregation. Consequently, we hypothesized that the combined effects of heat and elevated pressure would facilitate the re-hydration of highly aggregated proteins in the tissue surrogate, greatly improving protein solubilization while simultaneously reversing protein-formaldehyde adducts and cross-links. OMICS Group International is one of the leading Open Access Publishers which is publishing 700+ peer-reviewed journals with the support of 50,000+ editorial board members as editorial team and aimed to disseminate the scholarly knowledge to the scientific society. OMICS Group also organizing 3000+ International Scientific Conferences and events yearly all over the world with the support of 1000+ Scientific associations worldwide. Citation: Fowler CB, O’LearyTJ, MasonJT (2014) Improving the ProteomicAnalysis of Archival Tissue by Using Pressure-Assisted Protein Extraction:A Mechanistic Approach.J Proteomics Bioinform7: 151-157."

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Last date updated on April, 2024