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We have developed an easy-to use methodology for refining large extensible markup language (XML) - based proteomics dataset with a high stringent and simple approach using VBA- coded plug-in. A methodology we term it (All and None). Selections of targeted candidates differentially significant between compared groups were selected based on its appearance or absence followed by peptide screening with a novel and simple approach. By testing the reliability and efficiency of this method, All and None was confirmed to be an applicable process for initial screening of biological biomarkers in complex specimens and tissue extract.
Citation: Magdeldin S, Yoshida Y, Zhang Y, Xu B, Yaoita E, et al. (2011) "All and None" Refining Strategy; Fishing Your Correct Protein from Proteomics Ocean. J Proteomics Bioinform 4: 123-124. doi:10.4172/jpb.1000178
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