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The molecular genetics of searching candidate gene on high-attitude adaptability and resistance to cold has become a worldwide hot topic in plateau biology, especial in human and yak. APEX1 as a reference gene, playing a crucial role in Base Excision Repair (BER) enzyme, researches about yak APEX1 can help to understand the adaption of yak in plateau, and then further to increase its economic value. Full length cDNA sequence of apurinic/ apyrimidinicsite endonuclease 1 (APEX1) was cloned from Tianzhu white yak to study the roles of APEX1 and its mechanisms in stress resistance. PCR and RACE techniques were used to obtain the full length of APEX1 cDNA sequence.
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