Diethylaminoethyl (DEAE) cellulose adsorbed Pointed Gourd Peroxidase (PGP) was employed in decolorization of synthetic dyes. The expressed activity of immobilized preparation on fifth repeated use was ~50% and decolorization achieved for synthetic dyes DR19 and dye mixture (DR19+DB9) was 64.9% and 61.5% respectively. Immobilized enzyme could effectively decolorize up to 88.2% and 77.4% of DR19 and dye mixture respectively in stirred batch process at 40°C whereas dye color removal monitored at 30°C and 50°C was comparatively low under similar conditions. Immobilized enzyme in the packed column used for the continuous removal of dye color could successfully decolorize DR19 and dye mixture to 69.4% and 51.4% after 50 d of operation. Thus, DEAE immobilized PGP is a simple, economical and effective preparation to remove color of synthetic dyes.
Citation: Jamal F, Goel T (2014) Diethyl Aminoethyl Cellulose Immobilized Pointed Gourd (Trichosanthes dioica) Peroxidase in Decolorization of Synthetic Dyes. J Bioprocess Biotech 4:187 doi: 10.4172/2155-9821.1000187