Some biofertilizers agents i.e. Rhizobium leguminosarum var. fabae, Bacillus megaterium var., phosphaticum and Trichoderma harizianum play important roles in enhancing the plant growth and controlling several diseases i. e. root rot and stem canker disease. In this work, under greenhouse conditions, Rhizoctonia solani caused dampingoff and death of all faba bean seedlings, however the three tested microorganisms resulted good biocontol role against the pathogenic fungus and the application of T. harizianum gave the best result in this trend. The treatment of T. harizianum and R. leguminosarum var. fabae to the soil infested with Rhizoctonia solani showed significant increase in leaves number of faba bean plants compared with the untreated plants or which treated plants or which treated with Bacillus megaterium var., phosphaticum after 40 days from planting. Application of T. harizianum and R. leguminosarum var. fabae gave the best plant growth while the presence of the pathogenic fungus showed significant decrease in fresh weight, dry weight and nodules number on roots of faba bean plants. Also, polyphenols and antioxidants contents in the shoots and roots were decreased in the presence of the pathogen compared with the untreated plants. Application of R. leguminosarum resulted significant increase in the roots and shoots total nitrogen and protein.