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Lignocellulosic biomass represents a huge potential for energy production worldwide. However, it has not been harnessed for biofuel and biogas production at industrial scale due to the problems in digestibility of the lignocellulosic materials and the inaccessibility of the polymers to the enzymes for degradation. There are various methods of pretreatment which can be classified into physical, physicochemical, chemical, and biological pretreatments. Efficient Cellulase is constantly required to work at higher temperature and pH for various industrial purposes. Efficient Cellulase is constantly required to work at higher temperature and pH for various industrial purposes. Bacillus has been improved genetically using gene technology where deletion of unnecessary enzyme resulted in many fold production of enzyme. Beside these Protein-engineered variants of the B. liquefaciens and B. halodurans for α-amylases were commercialized for its improved alkali tolerance.