Monosaccharide characterization study can give valuable information on newly discovered intact mucopolysaccharide. The compound fucosylated chondroitin sulfate (FuCS) is a unique mucopolysaccharide having various bioactive properties reported only in targeted holothurians species. The monosaccharide composition of FuCS from a sea cucumber, Holothuria arenicola was studied by comparing a modified and conventional 1-phenyl- 3-methyl-5-pyrazolone (PMP) derivatization method where tributylamine addition was found to improve the chromatographic conditions. The monosaccharide proportion of FuCS from H. arenicola was determined to be 1:1:0.44 for glucuronic acid: N-acetylgalactosamine: fucose, respectively. Thus, the FuCS monosaccharide ratio of H. arenicola was first reported and this method is more useful for structurally similar compound analysis.
Citation: Myron P, Siddiquee S, Azad SA, Yong YS. (2015) Tributylamine Facilitated Separations of Fucosylated Chondroitin Sulfate (Fucs) by High Performance Liquid Chromatography (HPLC) into its Component Using 1-Phenyl- 3-Methyl-5-Pyrazolone (PMP) Derivatization. J Chromatogr Sep Tech 6:256. doi: 10.4172/2157-7064.1000256