Random integration is a phenomenon in which transfected DNA molecules integrate into (random sites of) the host genome via non-homologous recombination. DNA Topoisomerase-IIa (Top2α) and reactive oxygen species (ROS) are responsible for causing genomic DNA damage that leads to random integration. Treating cells with Top2α siRNA significantly reduces random integration frequency, while the absence of Top2β had little or no impact.

Citation: Kamekawa H, Kurosawa A, Umehara M, Toyoda E, Adachi N (2013) Endogenous Factors Causative of Spontaneous DNA Damage that Leads to Random Integration in Human Cells. Gene Technology 2:105.

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