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ELISA Technique:

This study was carried on 70 subjects classified into the following groups: Group 1: Include 50 patients with suspected cow milk protein allergy. Diagnosed by presence of chronic diarrhea with history of recent introduction of cow milk and positive elimination test. Group 2: Include 20 age and sex matched apparently healthy subjects, Their ages were ranged between (8-18) months.All individuals included in this study were subjected to full history taking, clinical examination, , complete blood count and determination of serum total Ig E , lactoglobulin and lactalbumin specific IgE which were carried out by ELISA technique.

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