A case control study was done. Eyes of 296 computer users were compared to 324 eyes of age and sex matched controls. A questionnaire of symptoms of dry eye (Dry eye scoring system (DESS)) was administered to both groups; assessed on a scale of 0-18, higher scores represent dry eye severity. Cells for impression cytology were harvested from inferior bulbar conjunctiva using 0.22 microns cellulose acetate filter paper. After staining with periodic acid- Schiff (PAS), slides were counterstained with hematoxylin and eosin.