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Improved grain quality is a major breeding target in rice (Oryza sativa L. subsp. japonica), owing to market demand. Rice cultivars grown in Hokkaido (42-45°N), the northernmost region of rice paddy cultivation in Japan and near the northern limit of rice cultivation, have been bred for over 100 years for adaptation to low temperature together with high yield and grain quality. In this study, for seven closely related rice cultivars released in Hokkaido in the last 70 years, we investigated the transcriptome profiles of developing seeds 8 days after flowering (DAF, middle stage) and 15 DAF (late stage) under natural conditions in Hokkaido (42.52°N) using a whole-genome oligonucleotide microarray. The transcriptome profiles were divided into two groups depending on stage and were more variable at the late than that at the middle stage.
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