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A sensitive and selective biosensor was constructed for detection of Salmonella typhimurium carrying SSeC gene, based on covalently coupling of molecular beacons (MBs) stained with daunorubicins (DNR) to single-walled carbon nanotubes (SWNTs) through EDC/Sulfo-NHS chemistry. In the absence of target, the fluorescence of daunorubicin was fairly week as result of dual fluorescence quenching. On the contrary, the daunorubicin was competed from the beacon due to the target-induced formation of rigid structure between the loop structure of the MB and the target sequence, which resulted in a decrease in the effect of dual fluorescence quenching, thereby the fluorescence intensity increased substantially. The target quantum was achieved by fluorescence increment.
A Novel Biosensor for Detection of Salmonella typhimurium Carrying SSeC Gene
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