Stem cells represent promising therapeutic potential in regenerative medicine. Several untreatable human diseases could be treated by the use of different types of stem cells. While stem cells hold great promise for biomedical research, the in vitro propagation and maintenance of these cells in an undifferentiated state is essential. Stem cell culture remains a challenge, even with the knowledge that scientists have gained over the past decade. Different types of stem cells often require different culture conditions. For example, human induced pluripotent stem cells (iPSC) and humanembryonic stem cells (hESC) must be cultured on coated plates with a supporting layer of feeder cells, such as mouse embryonic fibroblasts (MEF) or human foreskin fibroblasts (HFF), or on an extracellular matrix, such as a basement membrane gel.