Bacteria of the Bacillus genus possess a great potential as probiotic cultures. Bacillus bacteria are among the most widespread microorganisms in nature. These bacteria are known to be producers of more than 200 antibiotics. Bacillus antibiotics differ in their structure, as well as spectrum of activity. Some strains of Bacillus synthesize bacteriocines, which are only effective against bacteria of the same species, others produce antibiotics against Gram-negative bacteria and still other strains have a wide spectrum of antibiotic activity (including antifungal and antiprotozoan). Thus, it is possible to find strains with unique spectrum of activity among Bacillus bacteria. The aim of this work was to isolate and characterize Bacillus strains with pronounce activity against food borne pathogens.