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Infection with hepatitis B virus (HBV) can be prevented by vaccination with HB surface antigen, which induces HBS specific antibodies and T cells. But the immunization status of workers and its relationship with occupational factors are not well documented. The goal is to examine the factors of risk and the immunization status against HBV among a population of workers. Methods: An assessment of the vaccination and the immunization status against HBV was conducted among a miscellaneous population of French workers, recruited from a medical occupational center, during a cross-sectional study.