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Many targeting signals in the plant secretory pathway have been identified but mechanisms of protein storage and of tissue- and speciesspecific accumulation strategies are not yet fully understood [1-3]. Two vacuolar reporters derived by the GFP fusion in frame with the C-terminal Vacuolar Sorting Determinant (VSD) of tobacco chitinase (GFPChi) and the N-terminal fusion in frame with the sequencespecific VSD of the cysteine protease barley Aleurain (AleuGFP) have been used in several occasions to characterize vacuolar sorting and were recently used them to distinguish between the specific function of the two closely related Qc-SNARE SYP51 and SYP52 [4-6]. SYP51 appeared more essential to GFPChi sorting to the central vacuole while SYP52 appeared important for the transport of both markers [6]. We present here a more detailed analysis of SYP5 effect on fluorescent markers than that reported by De Benedictis [6] and further discuss the SYP5s effect on the last step of vacuolar sorting
For more information : Marianna F, et al.
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