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The recent RNA sequencing or RNA-Seq, involves highthroughput sequencing of short cDNA fragments obtained from the pool of RNA (total or fractionated, such as poly(A)+ or ribosomal rna depleted) to provide single-base resolution to the transcriptome. The advent of RNA-Seq enabled cataloguing transcripts of all species, including coding and non-coding mRNAs; to determine the transcriptional structure of genes, splicing patterns and other post-transcriptional modifications.
Citation: Gupta S (2015) Transcriptomics: Better Resolutions. Gene Technology 4:111.
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