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The objective of this research was to investigate the effect of carbon dioxide injection during extrusion process at different melt temperatures 95, 115 and 135°C on physiochemical properties and enzymatic saccharification of extruded corn starch for fermentation substrate preparation. Carbon dioxide gas was injected during extrusion at the flow rate of 500 ml/min and pressure of 3 MPa. For enzymatic saccharification, 0.8% α-amylase enzyme was used at different saccharification periods.
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