High-fat diets lead to obesity and metabolic disorders. The rs1761667 CD36 gene polymorphism may predict the preference for dietary fat. Aim: To determine the association of the CD36 gene polymorphism with fat intake and lipid abnormalities in subjects from West Mexico. Methods: In a cross-sectional study, 441 subjects were divided into normal weight, overweight and obese groups. Real-time PCR determined CD36 genotypes (AA, AG, and GG). Lipid biochemical tests and a 3-day food record were assessed. Results: The allele of CD36 was prevalent in 57.1% (n=252) of the total cases. The overweight A/A subjects had a significant higher intake of calories, protein, total fat, saturated fatty acids (SFA), monounsaturated fatty acids (MUFA) and polyunsaturated fatty acids (PUFA) than the other genotype carriers. Furthermore, high serum cholesterol levels were associated with the A/A genotype than to the A/G genotype carriers (OR=2.75, CI 1.33-5.69; p=0.005). Conclusions: The allele of CD36 was predominant in subjects from West Mexico. In addition, a high-fat diet and high serum cholesterol levels were associated with the A/A genotype.