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Bovine brucellosis is a zoonosis known to be a major public health hazard of great economic importance worldwide. Its detection in cattle is frequently done using serological tests like Rose Bengal Plate Test (RBPT) and Competitive- Enzyme Linked Immuno Sorbent Assay (C-ELISA). Few studies have been conducted to compare the serological tests most commonly used to detect bovine brucellosis in Rwanda.