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DNA delivery to cells has gained much attention as a powerful approach in gene therapy for treatment of inherited and acquired diseases. The transfection process depends on the nature of the binding interaction between DNA components of the non-viral delivery vector. The aim of this review is to explore the use of isothermal titration microcalorimetry (ITC) in the investigation of thermodynamic binding interactions between DNA and cationic components essential for DNA targeting to the cells. The review will focus on the interaction of DNA with block copolymers, surfactants and cationic lipids.
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