Acute bacterial conjunctivitis and their complications are commonly treated by 6 mg/mL ticarcillin and 50 mg/mL vancomycin eyes drops, formulated separately in hospitals. A rapid, selective and specific LC-tandem MS method has been developed and validated for simultaneous quantification of ticarcillin and vancomycinin solution and in presence of degradation products. Atlantis® C18 Column T3 (250×4.6 mm I.D., 5 μm) and gradient mobile phase composed of 44 mM trifluoracetic acid/methanol allowed for good separation. Degradation products were separated on LC and detected with mass spectrometry in full scan mode. The method was suitable forthe stability study of ticarcillin-vancomycin pH 5.2 solutionsover an 8-day period storage at 5 ± 3°C and at room temperature (25 ± 2°C). The solutions remained stable up to 5 days at 5 ± 3°C. At room temperature, degradation of ticarcillin was accelerated in presence of vancomycin, while vancomycin stability was not further affected by ticarcillin. High-resolution mass spectrometry was used for the characterization of the degradation products.
Belissa E, Nino C, Bernard M, Henriet T, Sadou-Yaye H, et al.(2014) Liquid Chromatography–Tandem Mass Spectrometry for Simultaneous Determination of Ticarcillin and Vancomycin in Presence of Degradation Products.Application to the Chemical Stability Monitoring of Ticarcillin-Vancomycin Solutions.J Chromatogr Sep Tech 5:243. doi: