In this study, data mining approach was used to generate association rules for predicting average flexibility from the various derived sequence and structural features. 21 parameters were calculated and their variable importance was calculated for 115 sequences of AGC kinase family belonging to mouse and human using Classification and Regression Tree (CART). Beta turns were found to have maximum influence on average flexibility while the total beta strands were found to exert minimum impact on average flexibility. Understanding the variable importance will prove useful as a simple pr edictor of flexibility from an amino acid sequence. This will aid in better understanding of phenomenon underlying the average flexibility and thus, will pave a way for rational design of therapeutics.
Citation: Amit KB, Neelima A, Varakantham P, Murty USN (2008) Exploring the Interplay of Sequence and Structural Features in Determiming the Flexibility of AGC Kinase Protein Family : A Bioinformatics Approach. J Proteomics Bioinform 1: 077-089.