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Intra Cytoplasmic Sperm Injection (ICSI) has been widely used since 1992 in the era of assisted reproduction Although this method has become the preferred technique for male infertility, total fertilization failure or low fertilization rates are still the problem that has to be solved. Many studies have focused on this problem and it was revealed that more than 80% of unfertilized oocytes were arrested at the metaphase II stage, possibly due to failed oocyte activation.
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