alexa Direct Measurement of Lipase Inhibition by Orlistat Using a Dissolution Linked In Vitro Assay

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Direct Measurement of Lipase Inhibition by Orlistat Using a Dissolution Linked In Vitro Assay

Purpose: To develop a bio-assay that would be able to directly test gastrointestinal and/or dissolution samples to determine lipase activity and inhibition by Orlistat. Methods: Enzyme assays were performed with porcine pancreatic lipase and para-Nitrophenyl Palmitate (pNPP) in pH 8.0 reaction buffer at 37°C. Substrate hydrolysis was monitored by absorbance changes at 410 nm. The dissolution of two Orlistat formulations was tested with a USP II apparatus. Samples were HPLC analyzed to determine release profile in addition to being diluted and directly assayed for inhibitory effect.

Citation: Lewis DR, Liu DJ (2012) Direct Measurement of Lipase Inhibition by Orlistat Using a Dissolution Linked In Vitro Assay. Clinic Pharmacol Biopharm. 1:103. doi: 10.4172/2167-065X.1000103

 
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